abstract：:Procedures are described for lesioning two components of the hippocampal formation (hippocampus, subiculum) using multiple injections of small amounts of ibotenic acid (IBO). The resulting loss of cells is more selective and limited than can be obtained with conventional techniques. Thus, problems associated with dama...

journal_title：Journal of neuroscience methods

authors： Jarrard LE

更新日期：1989-09-01 00:00:00

abstract：:A method by which muscles, nerves, and a section of spinal cord from a turtle can be stably maintained in vitro for many days has been developed. Tests of the viability of central and neuromuscular synapses, and the histochemical properties of muscle fibers have indicated that the preparation is viable and shows littl...

journal_title：Journal of neuroscience methods

authors： Lennard PR,Boss V,Shirley KA

更新日期：1989-08-01 00:00:00

abstract：:Precisely localized lesions in cuticular structures of insects can be produced by fine glass rods, vibrating transversally at frequencies above 100 kHz. The oscillating edge of the glass rod scrapes off the chitinous material at minimal elastic deformation during treatment. The achieved roughness height is in the 0.1 ...

journal_title：Journal of neuroscience methods

authors： Gödde J

更新日期：1989-07-01 00:00:00

abstract：:The fluorescent dye Fluorogold has been recently introduced as a retrograde neuronal tracer that can be used in combination with immunohistochemistry. It has, however, lately been reported that immunohistochemical processes induced rapid photobleaching of Fluorogold. In the present study we prove that it is the water ...

journal_title：Journal of neuroscience methods

authors： Ju G,Han ZS,Fan LZ

更新日期：1989-07-01 00:00:00

abstract：:Motorized linear slides used as micromanipulators for biological use suffer from 3 problems: vibration at low speed, poor ergonomic design of the controller and slow coarse positioning. These problems are not hard to solve by minor modifications to the controller and the slides. ...

journal_title：Journal of neuroscience methods

authors： Gao XQ,Sachs F

更新日期：1989-06-01 00:00:00

abstract：:The use of fluorescent calcium indicator, Indo-1, was evaluated for measuring changes in intracellular free calcium during electrical stimulation and anoxia in hippocampal slices. Fluorescence was measured from slices illuminated with brief (3 ns) light pulses (337 nm wavelength) from a nitrogen laser. This method of ...

journal_title：Journal of neuroscience methods

authors： Sick TJ,Rosenthal M

更新日期：1989-05-01 00:00:00

abstract：:Mammalian brain glycogen is adequate to support oxidative metabolism for several minutes. The present studies were done primarily to develop the guinea pig hippocampal slice as a model for studying the function and regulation of that glycogen. Slice glycogen falls to 6 nmol/mg dry wt. during the first hour of incubati...

journal_title：Journal of neuroscience methods

authors： Lipton P

更新日期：1989-05-01 00:00:00

abstract：:A simple method of generating precisely defined pulsed linear acceleration stimuli was developed and used to study vestibular responses to cranial acceleration. Electromechanical shakers were coupled to a stimulus platform. The platform, in turn, was used to couple stimuli to the skull. Movements were controlled by a ...

journal_title：Journal of neuroscience methods

authors： Jones TA,Schiltz T

更新日期：1989-03-01 00:00:00

abstract：:The tail-skin temperature is an important factor in determining tail-flick latency to noxious radiant heat in rats and mice. A simple, non-invasive method for recording the tail-skin temperature during conventional tail-flick testing is described. The method is conveniently performed during conventional tail-flick tes...

journal_title：Journal of neuroscience methods

authors： Tjølsen A,Lund A,Berge OG,Hole K

更新日期：1989-01-01 00:00:00

abstract：:Three-dimensional (3D) reconstruction from serial sections that are undistorted and true-to-life is only possible when a reference system exists that allows correct matching of the section drawings. This paper describes a simple method for producing reference lines that ensures proper alignment of the drawings. (1) Th...

journal_title：Journal of neuroscience methods

authors： Meyer EP,Domanico VJ

更新日期：1988-12-01 00:00:00

abstract：:A method is described which allows simultaneous recording of the activity of several neurons in the visual cortex of awake unrestrained kittens for a period sufficiently long to follow experience-dependent changes in receptive field properties. The electrodes consisted of 25 microns thick Teflon-coated platinum-iridiu...

journal_title：Journal of neuroscience methods

authors： Mioche L,Singer W

更新日期：1988-11-01 00:00:00

abstract：:I describe here the successful visualization of interhemispheric callosal connections in the live mammalian cortex. The development of this method was prompted by the finding that fluorescent tracer labeling of groups of cortical neurons, when done under optimal conditions, is sufficiently intense to be visible even i...

journal_title：Journal of neuroscience methods

authors： Malach R

更新日期：1988-10-01 00:00:00

abstract：:The effect of acute ethanol on adenosine content in four motor areas of the male Sprague-Dawley rat brain was investigated using HPLC-fluorescence detection. Since basal adenosine levels are difficult to assess due to extremely rapid turnover of adenosine, four different methods of sacrifice were also evaluated for ad...

journal_title：Journal of neuroscience methods

authors： Clark M,Dar MS

更新日期：1988-10-01 00:00:00

abstract：:A method is described enabling the recording of synaptic currents from an isolated interganglionic interneurone in the central nervous system of the cockroach, Periplaneta americana, using an oil-gap recording system. The circuitry contains a Wheatstone bridge in which the preparation is the unknown resistance. Using ...

journal_title：Journal of neuroscience methods

authors： Mony L,Hue B,Tessier JC

更新日期：1988-09-01 00:00:00

abstract：:Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Since the latter molecule has been conjugated to several histochemical markers, the use of biocytin as an intracellular marker was investigated. Electrodes were filled with a solution of 4...

journal_title：Journal of neuroscience methods

authors： Horikawa K,Armstrong WE

更新日期：1988-08-01 00:00:00

abstract：:Subunit B of cholera toxin was used as a tracer substance in the central nervous system after being injected into various brain regions, mainly somatosensory relay structures. The tracer was localized with an immunoperoxidase technique, using monoclonal antibodies raised in mouse hybridomas. This method, which is appl...

journal_title：Journal of neuroscience methods

authors： Ericson H,Blomqvist A

更新日期：1988-07-01 00:00:00

abstract：:The use of paper impregnated with Bromphenol blue for recording normal rat footprints is described. Because paper provides a better grip than X-ray film for the paws of the rats, the resulting tracks make manual scoring easier and more accurate. In addition, the cost of this technique is considerably lower. ...

journal_title：Journal of neuroscience methods

authors： Lowdon IM,Seaber AV,Urbaniak JR

更新日期：1988-07-01 00:00:00

abstract：:This article is part of a study on cortical microcircuitry in which flash evoked potentials (FEPs) were recorded from the anesthetized rat visual cortex. The FEPs were subjected to current source density analysis (CSD). One of the limitations of the CSD method is the need for recording sites outside the sampling volum...

journal_title：Journal of neuroscience methods

authors： Vaknin G,DiScenna PG,Teyler TJ

更新日期：1988-06-01 00:00:00

abstract：:Procedures are described for studying protein phosphorylation in 1 mm diameter micro-slices of rat brain tissue using two-dimensional electrophoresis as analytical tool. The activity of several protein phosphorylating systems, including a major system phosphorylating a 40 kDa substrate complex, was highly dependent on...

journal_title：Journal of neuroscience methods

authors： Rodnight R,Zamani R,Tweedale A

更新日期：1988-05-01 00:00:00

abstract：:A method is described for producing electrolytically sharpened and lacquer-insulated steel microelectrodes and for their attachment to a multibarrel micropipet assembly by light-curing bonding compound. This electrode complex is specially designed for recordings from deep brain structures. It permits the analysis of t...

journal_title：Journal of neuroscience methods

authors： Gottschaldt KM,Hicks TP,Vahle-Hinz C

更新日期：1988-04-01 00:00:00

abstract：:In the three-dimensional reconstruction of neuronal structure, it is imperative that ribbons of semithin or ultrathin sections be obtained. Resin-embedded semithin sections display better structural details than paraffin-embedded sections. The cutting and collecting of long ribbons of resin-embedded semithin sections ...

journal_title：Journal of neuroscience methods

authors： Wali N,Jagadeesh JM

更新日期：1988-03-01 00:00:00

abstract：:A flow rate monitor system for constant perfusion of media for maintaining physiological preparations, particularly in vitro brain slices, is described. The system consists of 3 components: the counter with a drip rate meter and alarm; a drop sensor; and a remote DC power supply. It provides accurate and immediate rea...

journal_title：Journal of neuroscience methods

authors： Rinaldi PC,Brown AM

更新日期：1988-02-01 00:00:00

abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...

journal_title：Journal of neuroscience methods

authors： Justice JB Jr,Nicolaysen LC,Michael AC

更新日期：1988-01-01 00:00:00

abstract：:In rats with unilateral 6-hydroxydopamine lesions of the substantia nigra apomorphine reliably induces a response of contralateral rotation. The present study shows that this response can readily be conditioned to a test environment which the rats are briefly exposed to during the onset of the apomorphine-induced rota...

journal_title：Journal of neuroscience methods

authors： Carey RJ

更新日期：1988-01-01 00:00:00

abstract：:A method which improves carbon fiber microelectrode selectivity for cationic amines by electrocoating the fiber with a thin film of the ionic polymer, Nafion, is described. The selectivity and response speed of these electrodes for the detection of electroactive cationic and anionic species found in brain extracellula...

journal_title：Journal of neuroscience methods

authors： Brazell MP,Kasser RJ,Renner KJ,Feng J,Moghaddam B,Adams RN

更新日期：1987-12-01 00:00:00

abstract：:Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verifica...

journal_title：Journal of neuroscience methods

authors： Myslinski N,Matthews B

更新日期：1987-11-01 00:00:00

abstract：:The glycogen-depletion technique has become a well-established method for determining histologically the cross-sectional distribution of a single muscle unit. A major drawback of this method is its low yield of one depleted unit per experiment. Furthermore, this technique is particularly unsuited for determining the l...

journal_title：Journal of neuroscience methods

authors： Chanaud CM,Pratt CA,Loeb GE

更新日期：1987-10-01 00:00:00

abstract：:Trace amounts of [14C]2-deoxyglucose (2-DG) were used to detect regions of the brainstem involved in forelimb stepping in thalamic and low spinal cats. Under ether anesthesia, cats were transected at the stereotaxic A12 level and T10 segment. Two hours later, 50 microCi/kg of 2-DG was infused i.v. and one of 4 procedu...

journal_title：Journal of neuroscience methods

authors： Shimamura M,Edgerton VR,Kogure I

更新日期：1987-10-01 00:00:00

abstract：:Some basic methodological issues concerning the use of the fluorescent tracers Fast blue (FB) and Diamidino yellow (DY) were studied using the projections of the red nucleus to the nucleus interpositus anterior (NIA) of the cerebellum of the cat. On standard Nissl-stained sections, it was possible to delineate 4 disti...

journal_title：Journal of neuroscience methods

authors： Condé F

更新日期：1987-09-01 00:00:00

abstract：:A semi-automated analysis system based on video image analysis was developed to count labelled and unlabelled nuclei of Schwann cells which had been exposed to tritiated thymidine followed by processing for autoradiography. A Model 3000 Image Analysis system (Image Technology Corporation, Deer Park, NY) was used to ac...

journal_title：Journal of neuroscience methods

authors： Baichwal RR,Yan L,Bosler A,DeVries GH

更新日期：1987-08-01 00:00:00

abstract：:Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...

journal_title：Journal of neuroscience methods

authors： Coates PW,Nathan RD

更新日期：1987-07-01 00:00:00

abstract：:Synaptic connections between retinal cells were studied by recording simultaneously from pre- and postsynaptic cells in the retinal slice preparation. The time course and waveform of the pre- and postsynaptic light responses were monitored, and the postsynaptic voltage responses to presynaptic current injection were m...

journal_title：Journal of neuroscience methods

authors： Wu SM

更新日期：1987-06-01 00:00:00

abstract：:After injection of 2-deoxyglucose (2-DG) labeled with tritium or carbon-14, autoradiograms were produced by thaw-mounting 4 micron frozen sections of rat brains on nuclear emulsion-coated slides. The results show that the distribution of radioactivity among different brain regions was similar and that the resolution a...

journal_title：Journal of neuroscience methods

authors： Duncan GE,Stumpf WE,Pilgrim C,Breese GR

更新日期：1987-06-01 00:00:00

abstract：:Details are described of techniques which allow the isolation of labelled, identified neurones suitable for patch clamp recording from the guinea pig spinal cord. Fluorescent labels, injected into either the hindlimb muscles or the cerebellum, are retrogradely transported to motoneurones or dorsal spinocerebellar trac...

journal_title：Journal of neuroscience methods

authors： Gibb AJ,Walmsley B

更新日期：1987-05-01 00:00:00

abstract：:In deeply anesthetized cats, determinations of motor-axonal conduction velocity (CV) were made using extracellular potentials recorded from single, functionally isolated motor axons innervating the muscle tibialis posterior. Axons were activated by suprathreshold electrical stimulation at the ventral-root level. Actio...

journal_title：Journal of neuroscience methods

authors： Gordon DA,Hamm TM,Enoka RM,Reinking RM,Windhorst U,Stuart DG

更新日期：1987-04-01 00:00:00

abstract：:Staining the brain for cytochrome oxidase (CO) produces patterns which can resemble the patterns produced by 2-deoxyglucose (2-DG) autoradiography. In order to assess the differences between CO patterns of long-term metabolic activity and 2-DG patterns of short-term activity, comparisons should, ideally, be made on th...

journal_title：Journal of neuroscience methods

authors： Silverman MS,Tootell RB

更新日期：1987-01-01 00:00:00

abstract：:We describe a family of 6 computer programs that measure, analyse and create graphic displays of complex motor patterns. The programs create lists of times at which successive bursts of impulses in different nerves started and stopped, and use these lists to calculate the periods and durations of these bursts and to c...

journal_title：Journal of neuroscience methods

authors： Mulloney B,Hall WM

更新日期：1987-01-01 00:00:00

abstract：:The usefulness of a number of new stereological principles for unbiased estimation of particle number and sizes and sampling of particles is illustrated together with a novel principle for unbiased estimation of anisotropic surfaces. The examples include descriptions of estimators of neurone and synapse number and siz...

journal_title：Journal of neuroscience methods

authors： Braendgaard H,Gundersen HJ

更新日期：1986-10-01 00:00:00

abstract：:Vertex analysis defines dichotomous networks in terms of the relative frequencies of pendant (Vp) and nodal (Vd) vertices. Va, Vb and Vc comprise the 3 classes of Vd which connect 2, 1 and 0 Vp respectively. The vertex ratio (VR) of Va/Vb may be used to define topology since Va and Vc approach equality in large networ...

journal_title：Journal of neuroscience methods

authors： Berry M,Sadler M,Flinn R

更新日期：1986-10-01 00:00:00

abstract：:Immunohistochemistry using antiserum against the basement membrane glycoprotein laminin, was shown to be an excellent marker for brain blood vessels. Throughout the brain of mice, rats, guinea pigs, monkeys and humans, the basement membrane of the vascular structures were strongly laminin-positive. The neuropil itself...

journal_title：Journal of neuroscience methods

authors： Eriksdotter-Nilsson M,Björklund H,Olson L

更新日期：1986-09-01 00:00:00